涡街信号处理程序，实现周期图谱法和中心校正相比较。-Vortex signal handler， Mapping method to achieve cycle

We propose a novel fluorescence emission difference microscopy (FED) system based on focusing cy- lindrical vector beams. In conventional FED, a Gaussian beam and a 0–2 π vortex phase plate are used to generate solid and hollow spots. We focus ra