使用三帧差法，对48孔板中的斑马鱼进行轨迹跟踪，最后绘制出每个孔板中的鱼的运动轨迹与运动距离。-Using three frame difference method, to the trajectory tracking of 48 orifice in zebrafish, finally draw out each orifice fish movement and movement in the distance.