thresholding_demo.m interactively loads an image stack, performs thresholding based on the Laplace operator and extracts time-series information from the image stack based on a binary mask provided by the thresholding operation.-thresholding_demo.m interactively loads an image stack, performs thresholding based on the Laplace operator and extracts time-series information from the image stack based on a binary mask provided by the thresholding operation.